dc.contributor.author Henry, S
dc.contributor.author Bovin, N
dc.date.accessioned 2012-05-13T20:31:05Z
dc.date.available 2012-05-13T20:31:05Z
dc.date.copyright 2008-09
dc.date.issued 2012-05-14
dc.identifier.citation Transfusion 2008; 48 (2S) Suppl.: 194A
dc.identifier.issn 0041-1132
dc.identifier.uri http://hdl.handle.net/10292/4136
dc.description.abstract Background: Red cells used in immunohematology assays are restricted by their natural phenotype and membrane characteristics. More recently a technology has been developed which allows for the attachment of a large variety of specific carbohydrates, peptides and other structures to the surface of cells – known as FSL cell surface coating (KODETM technology). FSL constructs are water dispersible and consist of three components; a F functional head group, a S spacer and a L diacyl lipid tail. This technology allows users to introduce novel antigen profiles or characteristics at the membrane surface of cells. Methods: FSL constructs were synthesised bearing carbohydrates, peptides, and fluorophores. One part (0.05 ml) of FSL construct 1 mg/ml and one part of packed red cells were combined, mixed and incubated at 37°C for 2 hours, washed and then analysed for the presence of constructs by direct-indirect agglutination and fluorescence serological methods. Results: In all cases red cells were shown to be able to be precisely labelled with serologically detectable FSL construct. Red cells were modified to carry ABO, H, Lewis, acquired-B, the animal antigen Galili, hyaluronic acid, fluorophores and blood group peptides (Miltenberger). Conclusion: FSL cell surface coating technology allows for the creation of red cells expressing controlled levels of normal and/or novel antigens or structures. Such cells can be used for quality control, specialised antibody screening and identification panels and have potential for novel future applications.
dc.publisher John Wiley & Sons
dc.publisher AUT University
dc.relation.uri http://onlinelibrary.wiley.com/doi/10.1111/j.1537-2995.2008.01891.x/abstract
dc.rights Copyright © 2008 John Wiley & Sons. All rights reserved. Authors retain the right to place his/her pre-publication version of the work on a personal website or institutional repository. This article may not exactly replicate the final version published in (please see citation) as it is not a copy of this record. An electronic version of this article can be found online at: (Please see Publisher’s Version)
dc.title The development of synthetic peptidolipids, glycolipids and other lipid-linked structures to create designer red cells
dc.type Conference Contribution
dc.rights.accessrights OpenAccess
aut.relation.endpage 195A
aut.relation.issue 2
aut.relation.startpage 194A
aut.relation.volume 48

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